Check your sequence (from ab1 file) is at the bottom (b). Move the cursor (a) to the bottom of the screen. Your sequence is located at the bottom of the alignment. Start EzEditor2 software and open the file that you have just downloaded. This file contains your sequences and reference sequences that show the highest sequence similarities. Click (c) to view the detailed result of “Identify”.Ĭlick (a) to download a data file for EzEditor2 tool. We will start the trimming process using a sequence alignment tool called EzEditor2. As you will see the chromatogram shown above, your sequence contains errors at the front end (as well as backend). This is cutoff is applicable for high-quality sequence only. The generally accepted 16S similarity cutoff is 98.7%. In this case, sequence similarity is 95.35% and you may be excited to discover a potentially new species. The hit species (a) is displayed with pairwise sequence similarity (b). Go to and paste your sequence as new query. Then, search through the EzBioCloud’s Identify. This file contains the result of the fairly good sequencing reaction. This contains a 16S sequence of a human fecal bacterium. If you used the sequence data without trimming the ends, your sequence will have a substantial amount of errors. Because of the sequencing chemistry, both ends of sequence contain substantial errors. Quality and manual inspection of the raw data is possible with the chromatogram.
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